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Electron cryo-microscopy (cryo-EM) is an integral part of the Ernst-Ruska Centre with world-leading equipment in ultra-high resolution electron microscopy.

Cryo-EM

Cryo-EM is an integral part of the Ernst-Ruska Centre with world-leading equipment in ultra-high resolution electron microscopy. The cryo-EM facility of the Ernst-Ruska Centre 3 is equipped with state-of-the-art cryo-microscopes 300 kV Titan Krios G4 (operational in Spring 2021) and 200 kV Talos Arctica including Gatan Bioquantum K3 detectors. For negative staining and cryo-EM, we use a Talos L120C from ThermoFisher Instruments. The facility is being maintained by Julio Ortiz and Thomas Heidler.


Talos Arctica operated at 200 kV. Samples are loaded by Julio Ortiz.

ER-C-3 Cryo-EMCopyright: Forschungszentrum Jülich/Ralf-Uwe Limbach


The Talos L120C microscope is suitable for analysis negative staining and cryo-samples.

ER-C-3 Cryo-EMCopyright: Forschungszentrum Jülich/Carsten Sachse


The latest Bioquantum K3 detector from Gatan.

ER-C-3 Cryo-EMCopyright: Forschungszentrum Jülich/Carsten Sachse



Vitrobot is used for standard plunge-freezing cryo-sample preparation.

ER-C-3 Cryo-EMCopyright: Forschungszentrum Jülich/Carsten Sachse


Additional Information

Head of ER-C-3

Structural Biology (ER-C-3)

Portrait


Prof. Dr. Carsten Sachse

Personal lab https://sachse.fz-juelich.de

Phone: +49 2461 61-2030
Fax: +49 2461 61-1448
E-Mail: c.sachse@fz-juelich.de

Team assistant: Melanie Hagen

Phone: +49 2461 61-2031
Fax: +49 2461 61-1448
E-Mail: m.hagen@fz-juelich.de